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The social amoeba Dictyostelium discoideum is a commonly used eukaryotic model organism for the study of cell division, chemotaxis, differentiation, phagocytosis, and other cellular processes. Electroporation is an effective and efficient method for delivering plasmid DNA into D. discoideum, an invaluable tool for studying intracellular processes. The technology is readily available but often prohibitively expensive. Although several custom-built electroporation devices have been developed, none deliver the specific 8.5kV/cm exponentially decaying waveform required for D. discoideum transformation. The present study examined whether a simple, inexpensive device can be built to produce this waveform through a simple resistor-capacitor (RC) circuit. A pulse generator RC circuit was built incorporating inexpensive electronic components and a 3D printed cuvette chamber. All four possible combinations of custom-built and commercial pulse generators and custom-built and commercial cuvette chambers were used to transform D. discoideum cells with a plasmid encoding green fluorescent protein (GFP). There were no significant differences in the number of surviving cells immediately following or 24 hours post-transformation between the systems. All combinations of custom-built and commercial systems achieved comparably high transformation efficiency shown by percent of cells expressing GFP six days after the transformation. Since the waveform-specific electroporation system we present here can be built by non-experts with easily obtainable materials and 3D printing, we envision this device to benefit investigators in areas with low research budgets and educators in multiple STEM fields. 

Deformable beamsplitters have been shown as a means of creating a wide field of view, varifocal, optical see- through, augmented reality display. Current systems suffer from degraded optical quality at far focus and are tethered to large air compressors or pneumatic devices which prevent small, self-contained systems. We present an analysis on the shape of the curved beamsplitter as it deforms to different focal depths. Our design also demonstrates a step forward in reducing the form factor of the overall system. 

Accommodative depth cues, a wide field of view, and ever-higher resolutions present major design challenges for near-eye displays. Optimizing a design to overcome one of them typically leads to a trade-off in the others. We tackle this problem by introducing an all-in-one solution - a novel display for augmented reality. The key components of our solution are two see-through, varifocal deformable membrane mirrors reflecting a display. They are controlled by airtight cavities and change the effective focal power to present a virtual image at a target depth plane. The benefits of the membranes include a wide field of view and fast depth switching.